The New Jersey Institute of Technology's
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Title:	Effect of 2,3-Butanedione monoxide on whole-cell potassium channel currents in rat single myocytes
Author:	Zhou, Youliang
View Online:	njit-etd1992-125 (viii, 47 pages ~ 1.3 MB pdf)
Department:	Biomedical Engineering Committee
Degree:	Master of Science
Program:	Biomedical Engineering
Document Type:	Thesis
Advisory Committee:	Engler, Peter (Committee chair) Kristol, David S. (Committee member) McArdle, Joseph J. (Committee member)
Date:	1992-05
Keywords:	Potassium channel Muscle cell Muscle contraction -- Regulation
Availability:	Unrestricted
Abstract:	The patch clamp technique is a simple procedure which can isolate ion channels on cell membranes. Our previous data have shown that 2,3-Butanedione Monoxime (BDM) could inhibit the calcium current by dephosphorylation of the channel. In this study, the effect of BDM on the potassium channels were investigated with the whole-cell recording method in single myocytes enzymatically isolated from the left ventricle of 3-4 months old rats. Superfusion of myocytes with BDM elicited a dose dependent decrease of the outward transient potassium current. BDM with concentrations of 5, 20 and 50 mM reduced the maximal peak potassium current by 7.3 ±2.3%, 68.7±6.1% and 100%. 5 µM of isoproterenol (Iso) partially reversed the inhibitory action of 20 mM BDM on potassium current. Furthermore, 2µM of isoproterenol (Iso) applied to bath solution without BDM could enhance the peak potassium current about 15%. Our present data suggest that BDM and Iso can either inhibit or enhance the potassium current by dephosphorylation or phosphorylation of the potassium channels.