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The New Jersey Institute of Technology's
Electronic Theses & Dissertations Project

Title: Differentiation of mouse embryonic stem cells into insulin-producing cells: optimization to enhance the overall differentiation efficiency
Author: Ma, Xiaotang
View Online: njit-etd2015-075
(xii, 81 pages ~ 2.0 MB pdf)
Department: Department of Biomedical Engineering
Degree: Master of Science
Program: Biomedical Engineering
Document Type: Thesis
Advisory Committee: Lee, Eun Jung (Committee chair)
Arinzeh, Treena Livingston (Committee member)
Cho, Cheul H. (Committee member)
Date: 2015-05
Keywords: Embryonic stem cells
Insulin-producing cells
Availability: Unrestricted
Abstract:

Embryonic stem cells have the potential to differentiate into multiple cell types including insulin-producing cells (IPCs), which is becoming one of the promising cell sources for treating type 1 diabetes mellitus. However, in order to achieve functional stem cell-derived cells, it is important to generate more mature IPCs and to keep long-term viability post differentiation process. In this study, we varied several factors including different embryonic body culture conditions, digested cells seeding density and various coatings required for differentiation to optimize a previously established protocol to enhance the overall differentiation efficiency. Moreover, a three-dimensional in vitro collagen tissue culture system was prepared to provide a more physiological culture environment for stem cell-derived IPCs. Survivability of IPCs was examined under both static and flow conditions and low flow rate of 0.02 ml/min resulted in better survival of IPCs in in vitro three-dimensional tissues.


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