Electrospinning has been extensively used as an effective technique for the fabrication of submicron and nanometer scale polymeric fibers. In spite of technological advances in electrospinning, creating a highly aligned fiber structure is a tough target to be achieved because of the chaotic motion of the electrospinning jet. In this study, two different techniques have been adopted to form a highly aligned nanofibrous scaffolds. Using these methods highly aligned fiber yams and mats are produced from Cellulose Acetate (CA) in a mixed solvent of acetone and water. In both the techniques the nanofibers suspend over the gap in a highly oriented manner, and they can be easily transferred onto a substrate for further applications. Since the fibers generated by electrospinning are in micrometer and nanometer scale, it is very complex to isolate and manipulate an individual nanofiber. To address this issue, the technique that is used to fabricate nanofiber mat is incorporated with some modifications to isolate a single nanofiber.
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