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Title:	The growth, enrichment, and dehalogenating properties of pyrococcus GBD and desulfurococcus SY
Author:	Mueller, Siegfried Georg
View Online:	njit-etd1995-099 (xvi, 114 pages ~ 2.9 MB pdf)
Department:	Department of Chemical Engineering, Chemistry and Environmental Science
Degree:	Master of Science
Program:	Environmental Science
Document Type:	Thesis
Advisory Committee:	Armenante, Piero M. (Committee chair) Kafkewitz, David (Committee member) Trattner, Richard B. (Committee member)
Date:	1995-01
Keywords:	Microbial growth.
Availability:	Unrestricted
Abstract:	Recent investigations of volcanic smoker chimneys along the Atlantic Rift has lead to the discovery of hyperthermophilic bacteria. In particular, Pyrococcus GBD and Desulfurococcus SY were studied, optimizing growth conditions. This was achieved using elemental sulfur amendments, artificial sea water, and dehydrated marine broth. The microbes were then acclimated to enriched cultures containing 2,4,6 trichlorophenol in 0.0126 mM, 0.063 mM, and 0.126 mM amendments. The amended cultures showed dehalogenating properties and were subjected to a data qualifying program. This program indicated that the dehalogenation was a function of microbial gowth. A mass balance experiment showed that both microbes were able to reductively dehalogenate 0.5 mM 2,4,6 TCP to 2,6 DCP, 2 MCP, and finally phenol in a quantitative manner.