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The New Jersey Institute of Technology's
Electronic Theses & Dissertations Project

Title: Characterization of activated sludge immobilized in calcium alginate gel with respect to biodegradation of 2-chlorophenol and benzene
Author: Singh, Manjari
View Online: njit-etd1989-025
(vii, 51 pages ~ 4.1 MB pdf)
Department: Department of Chemical Engineering, Chemistry and Environmental Science
Degree: Master of Science
Program: Environmental Science
Document Type: Thesis
Advisory Committee: Sofer, Samir S. (Committee chair)
Trattner, Richard B. (Committee member)
Strom, Peter F. (Committee member)
Date: 1989
Keywords: Phenols--Biodegradation.
Benzene--Biodegradation.
Sewage--Purification--Activated sludge process.
Sewage--Microbiology.
Availability: Unrestricted
Abstract:

Activated sludges acclimated with phenol and 2-chlorophenol in one case, and a mixture of benzene, toluene, and o, in and p-xylene in another case, were characterized for biodegradation of 2-chlorophenol and benzene, respectively. Activated sludge was obtained from the Parsippany-Troy Hills Water Pollution Control Plant (NJ).

For both cases colonies were isolated before acclimation, after acclimation, and at the end of biodegradation studies. Since the unacclimated group was the same in both cases, there were a total of five different groups for which characterization was carried out.

For all the five groups together, a total of 54 isolates were obtained and 35 of these could be assigned to a genus. The genera assigned included Bacillus, Lactobacillus, Microbacterium, Plesiomonas, Kurthia, Kiebsiella, Corynebacterium and Pseudomonas.

Studies done with individual end run isolates and their mixture, in shaker flasks, established that the primary degrader of 2-chlorophenol was a Bacillus and that of benzene was a Pseudomonas.


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