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Title:	Detection and determination of N-Nitrosohexamethyleneimine in benzenesulfonamide, N-[[(hexahydro-1-H azepin-1-YL)amino]-carbonyl]-4 methyl by HPTLC
Author:	Valdez, Anulfo A.
View Online:	njit-etd1988-050 (vi, 40 pages ~ 8.2 MB pdf)
Department:	Department of Chemical Engineering, Chemistry and Environmental Science
Degree:	Master of Science
Program:	Chemistry
Document Type:	Thesis
Advisory Committee:	Greenberg, Arthur (Committee chair) Tomkins, R. P. T. (Committee member) Grow, James M. (Committee member)
Date:	1988-05
Keywords:	Nitroso-compounds Thin layer chromatography
Availability:	Unrestricted
Abstract:	A simple method, by High Performance Thin Layer Chromatography HPTLC), was developed for rapid determination of N-Nitrosohexamethyleneimine in Benzenesulfonamide, N-[((hexahydro-1 H Azepin-1-yl)amino]-Carbonyl]-4 methyl, (Tolazamide). Separation of N-Nitrosohe-xamethyleneimine from a Tolazamide solution in chloroform was obtained by precipitation of Tolazamide with n-heptane and by passing the resulting clear solution through a small silica column which was eluted with methanol. Determination of the N-nitroso compound was achieved by ultraviolet irradiation of it on activated High Performance Silica Gel Plates. Detection was performed by spraying the plates with triethylamine and fluorescamine reagent giving a fluorescent product. Visual detection limits of 25 ppb were observed. The method is recommended for rapid screening purposes.