The New Jersey Institute of Technology's
Electronic Theses & Dissertations Project

Title:	Separation technique on proteins via a pH-Parametric pump : a theoretical and experimental study
Author:	Pancharoen, Ura
View Online:	njit-etd1982-008 (xii, 334 pages ~ 9.3 MB pdf)
Department:	Department of Chemical Engineering and Chemistry
Degree:	Doctor of Engineering Science
Program:	Chemical Engineering
Document Type:	Dissertation
Advisory Committee:	Huang, Ching-Rong (Committee chair) Roche, Edward Charles, Jr. (Committee member) Abd-El-Bary, Mahmoud F. (Committee member) Kappraff, Jay (Committee member) Tassios, Dimitrios P. (Committee member)
Date:	1982
Keywords:	Proteins--Separation. Separation (Technology).
Availability:	Unrestricted
Abstract:	The separation of protein mixtures via pH-parametric pumping was investigated both theoretically and experimentally. A simple system consisting of one column packed either with cation or anion exchanger was first considered. A system of parapumps was then developed with more columns which were connected in series and packed alternately with cation and anion exchangers. Various methods of operation of parapumps are discussed. Enrichment and spliting of protein mixtures were examined. In most cases, the separation factor was defined at a steady state condition and was improved by increasing the number of cycles and columns. Computational methods for predicting both Batch and Semi-Continuous parametric pump performance, with equilibrium conditions described were developed. The physical system was characterized by means of interphase mass transfer rates. These methods were based on a set of exterior and interior material balances. Linear parameters were calculated for the adsorption of the solute on the ion exchanger. A mathematical model based on elementary matrix algebra was developed as well as a graphical method. The properties of eigenvalues and eigenvectors of this formalism were studied. The subject was extended to more complex situations involving a multi-column, and the separation of multi-protein. There is good agreement between predicted and experimental results.