The New Jersey Institute of Technology's
Electronic Theses & Dissertations Project

Title:	An experimental study of comparison between pH-parametric pumping and pH-cycling zone adsorption process on protein separation
Author:	Jajalla, Vincent P.
View Online:	njit-etd1981-013 (viii, 80 pages ~ 13.0 MB pdf)
Department:	Department of Chemical Engineering and Chemistry
Degree:	Master of Science
Program:	Chemical Engineering
Document Type:	Thesis
Advisory Committee:	Huang, Ching-Rong (Committee chair) Abd-El-Bary, Mahmoud F. (Committee member) McCormick, John E. (Committee member)
Date:	1981-09
Keywords:	Proteins -- Separation Separation (Technology)
Availability:	Unrestricted
Abstract:	In this thesis, an experimental investigation was conducted to compare the performance of the continuous pH-parametric pumping and the pH-cycling zone adsorption process on protein separation. The model system studied was hemoglobin-water. on CM-Sepharose CL-6B (cation) exchanger in a single-column system. Various modes of operation were tried on both the pH-parametric pumping and the pH-cycling zone adsorption method. The separation factors for each mode of operation were computed and compared. It is shown that the continuous pH-parametric pumping, with circulation to insure the pH uniformity inside the column, has the better separation factor than the pH-cycling zone adsorption method.