The New Jersey Institute of Technology's
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Title:	Continuous fractionation of protein mixtures by pH-parametric pumping
Author:	Wong, Yuk-Wei
View Online:	njit-etd1979-016 (vii, 66 pages ~ 1.8 MB pdf)
Department:	Department of Chemical Engineering and Chemistry
Degree:	Master of Science
Program:	Chemical Engineering
Document Type:	Thesis
Advisory Committee:	Chen, Hung T. (Committee chair) Lei, George Y. (Committee member) Lewandowski, Gordon (Committee member)
Date:	1979-06
Keywords:	Separation (Technology) Proteins
Availability:	Unrestricted
Abstract:	Continuous pH-parametric pumping separations of a haemoglobin - albumin - CM Separose system were experimentally investigated. The parapump system has a feed, containing the protein mixture to be separated, introduced alternately to the top and bottom of the chromatographic column. The top and bottom products are withdrawn from the apparatus during the bottom and top feed respectively. It is shown that under certain conditions the pH-driven parametric pump has the capacity for removal of a protein component from one product fraction and large enrichment in the other fraction. Moreover, the continuous process can be operated with a large feed throughput.