The New Jersey Institute of Technology's
Electronic Theses & Dissertations Project

Title:	A study on the growth and asparaginase production of vibrio succinogenes in a chemostat
Author:	Krautheim, Preston David
View Online:	njit-etd1979-003 (x, 138 pages ~ 6.4 MB pdf)
Department:	Department of Chemical Engineering and Chemistry
Degree:	Master of Science
Program:	Chemical Engineering
Document Type:	Thesis
Advisory Committee:	Greenstein, Teddy (Committee chair) Hanesian, Deran (Committee member) McCormick, John E. (Committee member)
Date:	1979
Keywords:	Vibrio succinogenes Asparaginase Enzymes Fermentation
Availability:	Unrestricted
Abstract:	A continuous fermentation assembly was constructed and studies on the growth of the organism, Vibrio succinogenes, and its production of asparaginase was conducted. Results were compared with that already performed on a batch system and showed that a continuous fermentation produced 31.83 I.U. enzymes/mM ammonium formate compared to 17.4 I.U. enzyme/mM formate for batch. Some of the runs performed indicated that the organism was washing out or giving very low specific growth rates. It was decided that this was not due to operational parameters of the chemostat, but possible chemical contamination in the medium. With all things considered, a continuous fermentation on producing the enzyme, asparaginase, was almost doubled when compared with that of batch making this form of fermentation a viable alternative.