The New Jersey Institute of Technology's
Electronic Theses & Dissertations Project

Title:	The separation of proteins via parametric pumping and ion exchange
Author:	Falcon, Paul Albert
View Online:	njit-etd1977-021 (vii, 118 pages ~ 3.4 MB pdf)
Department:	Department of Chemical Engineering and Chemistry
Degree:	Master of Science
Program:	Chemical Engineering
Document Type:	Thesis
Advisory Committee:	Chen, Hung T. (Committee chair) Hanesian, Deran (Committee member) Roche, Edward Charles, Jr. (Committee member)
Date:	1977-06
Keywords:	Separation (Technology) Ion exchange Proteins
Availability:	Unrestricted
Abstract:	A pH parametric pump, coupled with ion exchange, was used in an experimental investigation to separate a protein-water binary mixture. The model system consisted of a hemoglobin-water binary mixture. A pH gradient throughout the parametric pumping column was sustained with the use of hollow fiber devices incorporating phosphate buffer circulation. Data is presented for both batch and semi-continuous recuperative mode pH parametric pumping. Continuous pH parametric pumping for protein isolation has also been envisioned. No attempt has been made to optimize this newly devel-oped process; however, certain parameters are suspected of controlling separation, including buffer concentration, half cycle time, packed column height, and reservoir displacement volume. Results show that ratios of bottom product concentration to top product concentration can be sustained at approximately 2.0, using the semi-continuous mode.